Study of Tc-annexin V uptake in apoptotic cell models of Parkinson’s disease
نویسندگان
چکیده
Methods Tc-HYNIC-annexin V was prepared and its binding to apoptotic cell models of Parkinson’s disease was studied in vitro. Cellular models of Parkinson’s disease were produced by administering different concentrations of 1-methyl-4-phenylpyridinium to PC12 and SH-SY5Y cell lines. Cell apoptosis rates were analysed by flow cytometry. Annexin V was labelled with Tc by hydrazinonicotinamide (HYNIC). Cell binding studies were carried out using cellular models of Parkinson’s disease. Cell uptake studies were also performed after different levels of MPP + treatment, and the correlation between the degree of apoptosis and Tc-HYNIC-annexin V uptake was analysed.
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